Microfluidic Cell Isolation Performance Data for Cancer Cell Enrichment

An innovative microfluidic technique achieved cell processing rates as high as 7.5×10⁶ cells per second. The method, developed at UCLA, uses microscale vortices to separate larger cancer cells from smaller blood cells based on size without mechanical filters. Viable long-term culture was established using cells collected off-chip, suggesting utility for clinical and research applications.

Use Cases

• Benchmarking high-throughput cell isolation techniques based on the described processing rate of 7.5×10⁶ cells/s
• Designing label-free cell enrichment systems based on the size-based trapping mechanism using microscale vortices
• Evaluating off-chip cell viability for clinical applications based on the successful long-term culture results mentioned

Strengths

• Empirically determined critical particle/cell diameter and operational flow rate for trapping initiation
• Demonstrated separation of cancer cells from blood with a high processing rate of 7.5×10⁶ cells/s
• Used a simple single-layered microfluidic device, avoiding clogging mechanical filters

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Last update date is unknown; freshness unverified

Provenance

Source

University of California, Los Angeles

Collection Method

Empirical determination of critical parameters using a microfluidic device with parallel expansion-contraction trapping reservoirs.