E. coli Ribosomal RNA Dielectrophoresis Response Data for Biosensing

Experimental data from a study demonstrating the dielectrophoresis (DEP) manipulation of ribosomal RNA extracted from E. coli cells across a frequency range of 3 kHz to 50 MHz. The dataset, authored by G. Giraud from the University of Edinburgh, includes quantitative measurements of positive and negative DEP responses, characterized by specific dipole moments and polarizability values. These results represent the first demonstration of DEP for fast capture and release of rRNA units.

Use Cases

• Modeling the dielectrophoretic response of biomolecules based on the reported Clausius–Mossotti model parameters.
• Designing microfluidic rRNA capture devices based on the frequency-dependent positive and negative DEP behavior.
• Estimating the effective permittivity and surface conductance of rRNA in aqueous solutions for biosensor development.
• Investigating the relationship between rRNA structure and counterion binding based on the inferred open molecular structure.

Strengths

• Reports specific quantitative measurements, including an induced dipole moment of 3300 D and a polarizability of 7.8×10−32 F m² for positive DEP.
• Defines clear experimental frequency ranges for positive (3 kHz–1 MHz) and negative (above 9 MHz) DEP responses.
• Provides inferred biophysical properties, such as an effective permittivity of 78.5 ε0 and a surface conductance of ∼0.1 nS.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

University of Edinburgh

Collection Method

Experimental measurement using dielectrophoresis with interdigitated microelectrodes and total internal reflection fluorescence microscopy.