Mouse Liver Proteomics Data Comparing 2-ME/DMSO and IAA Workflows

6.7 MB of proteomics data compares a novel 2-mercaptoethanol/DMSO workflow against conventional iodoacetamide treatment for cysteine alkylation. The dataset, authored by Arisa Suto and last updated in May 2026, likely contains quantitative performance metrics from LC-MS/MS analysis of mouse liver proteomes and ovarian clear cell carcinoma samples.

Use Cases

• Benchmarking cysteine alkylation methods based on quantitative reproducibility metrics
• Analyzing proteome-wide sequence coverage improvements based on the described workflow
• Investigating cisplatin-induced protein alterations in ovarian cancer based on the application example

Strengths

• Includes specific performance metrics: peptide quantified CV ≤ 20% increased from 61.4% to 86.1%
• Describes a 1.6- to 1.9-fold increase in cysteine-modified peptides
• 77% of proteins exhibited improved sequence coverage with the optimized treatment

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment

Provenance

Source

figshare

Collection Method

Mouse liver proteomes processed with different alkylation workflows followed by LC-MS/MS analysis.

Freshness

Last updated 2026-05-08 06:46:59