35-Color Murine Immune Cell Phenotyping Panel for Cancer Models

A 35-color full spectrum flow cytometry panel for in-depth immune cell phenotyping from murine tumor and spleen samples. Data was acquired using a SONY ID7000 spectral analyzer and processed with autofluorescence extraction and spectral unmixing. The dataset, 525.8 MB in size, was uploaded by Aida Hansen and last updated on April 23, 2026.

Use Cases

• Profiling major immune cell populations and their subpopulations based on the 35-color panel design.
• Assessing levels of cell activation and exhaustion states in murine cancer models.
• Benchmarking spectral unmixing and autofluorescence extraction workflows using the described WLSM algorithm.
• Comparing immune landscapes between different murine tumor models like 4T1 and E0771.

Strengths

• High-parameter panel with 35 colors enabling detailed immune cell characterization from a single sample.
• Includes data from two distinct murine cancer models (4T1 and E0771) for potential comparative analysis.
• Specifies the acquisition instrument (SONY ID7000) and analysis software version (2.0.2) for reproducibility.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analytical needs.

Provenance

Source

figshare

Collection Method

Data acquired using SONY ID7000 spectral analyzer from full-stained single cell suspensions of murine tumors and spleen.

Freshness

Last updated 2026-04-23 20:46:39; freshness should be verified.