Mouse Hippocampus 3DEM Data for Synaptic Plasticity Analysis

3DEM dataset from Masaaki Kuwajima via the Texas Data Repository, last updated March 18, 2024. It contains 3DEM images and reconstructions from the hippocampal CA1 area of a mouse brain slice used to study long-term potentiation. The data includes unaligned tSEM images, aligned series for Reconstruct software, and a movie of a traced axon.

Use Cases

• Reconstructing 3D axon morphology based on the provided trace files and aligned image series.
• Analyzing synaptic ultrastructure changes associated with optically induced long-term potentiation.
• Developing or testing image alignment and segmentation algorithms for tSEM image stacks.
• Creating visualizations or educational materials based on the 3D reconstruction movie.

Strengths

• Dataset is structured into three distinct, purpose-specific parts for different analysis stages.
• Includes a processed 3D reconstruction movie, providing a direct visualization output.
• Data is associated with a specific experimental protocol for inducing synaptic plasticity.

Limitations

• Row count and total file size are unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Texas Data Repository Harvested Dataverse

Collection Method

3D electron microscopy (3DEM) acquired from a chemically fixed acute brain slice.

Time Range

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Freshness

Last updated 2024-03-18 10:04:45; freshness should be verified.

Geography

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