4-HNE induces cell death through the VCP-related ubiquitination pathway in diabetic cardio

A 4.5 MB dataset from figshare, authored by Renshan Liang and last updated in May 2026, under a CC-BY-4.0 license. It contains experimental data investigating the role of the lipid peroxidation product 4-HNE in diabetic cardiomyopathy. The study identifies 4-HNE binding to the VCP protein and explores the protective effects of metformin in both in vitro cardiomyocyte models and in vivo diabetic mouse models.

Use Cases

• Modeling the relationship between 4-HNE adduct formation and cardiomyocyte death based on described experimental results.
• Analyzing the effect of metformin on protein ubiquitination levels and cell survival in diabetic cardiomyopathy models.
• Investigating the VCP-related ubiquitination signaling pathway disruption mentioned in the study description.
• Studying the interaction between the Nrf2-GSTP1 pathway activation and 4-HNE-induced damage as described.

Strengths

• Includes both in vitro (H9C2 cardiomyocytes) and in vivo (HFD-STZ diabetic mice) experimental models as described.
• Data files are provided in accessible formats (DOCX, XLSX) totaling 4.5 MB.
• Uses mass spectrometry to identify specific protein adducts (4-HNE binding to VCP at K336).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale statistical analysis.
• The dataset is focused on a specific molecular mechanism, which may limit generalizability to other disease contexts.

Provenance

Source

figshare, author Renshan Liang.

Collection Method

Experimental data from mass spectrometry analysis, cell culture (H9C2), and animal models (HFD-STZ diabetic mice).

Time Range

Experimental timeframe not specified; dataset metadata updated in 2026.

Freshness

Last updated 2026-05-08 18:34:26; freshness should be verified.

Geography

Laboratory study; geographic origin of samples not specified.