HepaRG Cell Methyl-Donor Metabolite and DNA Methylation Data

28.1 KB of experimental data on the effects of a folate-choline compound on cellular metabolism and epigenetic markers. The dataset includes quantified levels of metabolites like S-adenosylmethionine (SAM) and DNA methylation measurements for five repetitive elements in human hepatic HepaRG cells treated with varying doses. It was authored by Kirsten Clement and last updated on May 30, 2026.

Use Cases

• Analyze dose-response relationships between folate-choline supplementation and metabolite levels based on quantified SAM, SAH, and methionine.
• Model epigenetic effects on specific genomic elements based on DNA methylation measurements for L1PA2, L1PA13, L1PREC1, Alu, and HERVK11I LTR.
• Investigate correlations between methyl-donor availability and gene expression changes based on BHMT and DNA methylation machinery transcript data.
• Validate in vitro hepatic cell models for studying nutritional epigenetics based on the HepaRG cell line experimental setup.

Strengths

• Includes specific quantitative results, such as a 71% increase in SAM at 40 nM and a 14-fold increase in HERVK11I LTR methylation.
• Reports detailed experimental conditions, including cell type (HepaRG), treatment doses (0, 1, 10, 40 nM), and duration (96 h).
• Employs precise measurement techniques like LC–MS/MS for metabolites and qRT-PCR for transcripts.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset's 28.1 KB size indicates a very limited scope, likely containing summary results rather than raw observations.

Provenance

Source

figshare

Collection Method

Experimental data from in vitro cell culture treatments, metabolite quantification by LC–MS/MS, and DNA methylation/transcript analysis.

Freshness

Last updated 2026-05-30 19:29:53; freshness should be verified.