A CRISPR-Cas9 knockout screen targeting 1,332 genes involved in protein degradation, metabolism, and vesicular trafficking. The dataset, authored by Zhan He and last updated in April 2026, identifies LAPTM4A as a key host factor regulating lysosome-autophagy homeostasis against PRRSV infection.
Use Cases
- Identify host factors critical for viral replication based on the CRISPR-Cas9 screen results.
- Study protein-protein interactions in host-virus systems based on the yeast two-hybrid screen identifying LAPTM4A-GP5 binding.
- Analyze ubiquitination and autophagic degradation pathways based on the described GP5-NEDD4-SQSTM1/p62 mechanism.
- Investigate transcriptional regulation of lysosomal genes based on the described TFEB nuclear translocation suppression.
Strengths
- Screen targets a specific and substantial set of 1,332 genes.
- Mechanistic insights are described in detail, linking viral protein interaction to cellular signaling cascades.
- Data is shared under a permissive CC-BY-4.0 license.
Limitations
- Row count is unknown, which may limit suitability assessment.
- Column-level documentation is absent; field semantics must be inferred after download.
- The dataset is relatively small at 7.9 MB, which may limit the scope of included raw data.
Provenance
- Source
- figshare
- Collection Method
- CRISPR-Cas9 knockout screen and yeast two-hybrid screen, as described.
- Time Range
- null
- Freshness
- Last updated 2026-04 23 03:00:05; freshness should be verified.
- Geography
- null