Molecular Assay Validation for Detecting Prorocentrum Cordatum in Brazilian Coastal Waters

A 2026 study by Andre Akira Gonzaga Yoshikawa presents a molecular workflow for detecting the harmful dinoflagellate Prorocentrum cordatum. The research validates PCR and LAMP assays targeting the ITS and psbA genes using water samples from Santa Catarina Island, Brazil. Field tests involved 48 samples, with detection limits ranging from 5.3 × 10² to 5.3 × 10⁶ cells per liter.

Use Cases

• Comparing sensitivity of PCR versus LAMP assays for field detection based on the described validation results.
• Developing rapid monitoring protocols for Prorocentrum cordatum based on the sample-to-result workflow.
• Assessing the specificity of molecular assays against related dinoflagellate species like P. lima and Takayama acrotrocha.

Strengths

• Detection limits are quantified for each assay (e.g., 5.3 × 10² cells l⁻¹ for ITS-PCR).
• Specificity was tested against three other potentially harmful dinoflagellate species.
• Field validation was performed on 48 water samples from Santa Catarina Island.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a 654.8 KB PDF, suggesting it is a research article rather than a structured data file.

Provenance

Source

figshare

Collection Method

Water samples were concentrated from small volumes (5–50 ml) using syringe filters and membranes, followed by molecular assay development.

Freshness

Last updated 2026-05-15 09:45:17; freshness should be verified.

Geography

Santa Catarina Island, southern Brazil.