Summit Wheat Genome Assembly for Prolamin Gene Characterization

Maria G. Rottersman produced a de novo genome assembly for the Summit wheat cultivar using ~398 Gb of PacBio HiFi sequencing data. The assembly comprises 21 pseudomolecules totaling 14.52 Gb, with an additional 79 Mb of unplaced scaffolds. It includes manual curation of 78 complete prolamin genes and 67 putative pseudogenes, deposited in NCBI under BioProject PRJNA1438777.

Use Cases

• Mapping prolamin gene deletions based on the manually curated gene list.
• Comparative genomics using the Summit cultivar assembly against the Chinese Spring reference.
• Designing breeding strategies for low-immunogenicity wheat based on identified gene clusters.

Strengths

• Genome assembly is based on ~398 Gb of PacBio HiFi sequencing data, suggesting high base-level accuracy.
• Manual curation identified 78 complete prolamin genes across specific subfamilies (e.g., 5 HMW-GS, 35 α-gliadins).
• Assembly is scaffolded into 21 pseudomolecules totaling 14.52 Gb, providing chromosome-level context.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• The dataset size is listed as 0.0 B, indicating the actual data files must be retrieved from the linked BioProject.

Provenance

Source

figshare, author Maria G. Rottersman.

Collection Method

De novo genome assembly from PacBio HiFi sequencing, scaffolded using the Kariega cultivar genome as a reference.

Freshness

Last updated 2026-05-22 06:55:41; freshness should be verified.