A Global Ligandability Map of Tryptoline Butynamide Stereoprobes Identifies Covalent Inhib

A dataset from figshare, last updated on 2026-05-21, compares the proteome-wide reactivity of stereochemically defined acrylamide and butynamide compounds built from a tryptoline core. The data, generated by Yijun Xiong, likely contains ligandability maps identifying proteins preferentially engaged by butynamides in human cancer cells, including the actin maturation protease ACTMAP. The findings support reactive group diversification for expanding the ligandability of the human proteome.

Use Cases

• Comparing proteome-wide reactivity of acrylamide versus butynamide reactive groups based on the stereoprobe ligandability maps.
• Identifying novel protein targets for covalent inhibitors based on butynamide-preferring proteins in cancer cells.
• Studying stereoselective inhibition of the actin maturation protease (ACTMAP) based on the identified tryptoline butynamides.
• Expanding the known ligandable cysteines in the human proteome based on the reactive group diversification strategy.

Strengths

• Dataset is structured in an XLSX file, a widely accessible tabular format.
• The description provides a specific biological context and discovery (ACTMAP inhibition) for the ligandability data.
• File size is 773.6 KB, indicating a focused and manageable dataset.

Limitations

• Row count and specific column definitions are unknown, requiring manual inspection after download.
• The dataset's scale is limited (773.6 KB), suggesting it may contain summary or focused results rather than raw proteomic data.
• Column-level documentation is absent; field semantics must be inferred from the description or after download.

Provenance

Source

figshare

Collection Method

Data likely generated through activity-based protein profiling (ABPP) experiments comparing covalent stereoprobes in human cancer cells.

Freshness

Last updated 2026-05-21 09:25:29; freshness should be verified.