New C-23-Methylated Steroid from Penicillium herquei WY-25

A research article describes the isolation and characterization of a new steroid and seven known analogues from the endophytic fungus Penicillium herquei WY-25, found in the plant Huperzia serrata. The structure was determined using HRESIMS, IR, UV, NMR, and single-crystal X-ray diffraction, and one compound showed inhibitory activity on nitric oxide production. The dataset, authored by Bei-Bei Zhang and last updated in May 2026, consists of a 21.4 MB DOCX file.

Use Cases

• Identifying novel steroid structures based on spectroscopic and crystallographic data.
• Screening for anti-inflammatory compounds based on nitric oxide inhibition assays.
• Studying the biosynthetic pathways of C-23-methylated steroids in endophytic fungi.
• Comparing metabolite profiles of fungi cultured in different media based on LC-MS profiling.

Strengths

• Compound 4 exhibited a specific IC50 value of 6.88 ± 0.22 μM in a bioactivity assay.
• The absolute configuration of the new steroid was determined by single-crystal X-ray diffraction.
• The production of compound 1 was consistently observed across varied culture media.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a single 21.4 MB document, limiting direct computational analysis.

Provenance

Source

figshare

Collection Method

Isolation and characterization from fungal culture.

Freshness

Last updated 2026-05-10 16:57:52; freshness should be verified.