PAlibDIA: N-Phosphoproteome Profiles for Nasopharyngeal Carcinoma

A 2026 dataset from figshare contains profiles of noncanonical protein N-phosphorylation sites in nasopharyngeal carcinoma. Author Yuqiu Wang developed a potassium phosphoramidate-based strategy and data-independent acquisition mass spectrometry to identify these sites. The dataset includes 493 pHis, 714 pLys, and 557 pArg sites, with 85% being novel discoveries.

Use Cases

• Identify novel therapeutic targets based on differentially regulated N-phosphosites associated with RNA splicing and chromatin remodeling.
• Benchmark new phosphoproteomics analysis methods against the reported PAlibDIA strategy for coverage and reproducibility.
• Investigate the role of noncanonical phosphorylation in tumorigenesis and cell signaling pathways specific to nasopharyngeal carcinoma.

Strengths

• Dataset reports 1,764 total N-phosphosites (493 pHis, 714 pLys, 557 pArg), providing a specific quantitative scope.
• Method validation demonstrates superior coverage and quantification reproducibility compared to conventional DDA and direct DIA approaches.
• 85% of the identified sites are reported as novel, indicating significant new biological findings.

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale statistical analysis.
• Column-level documentation is absent; field semantics must be inferred after download.
• The 14.2 KB file size suggests a highly summarized or metadata-focused dataset rather than raw spectral data.

Provenance

Source

figshare, author Yuqiu Wang.

Collection Method

Generated using a potassium phosphoramidate-based chemical phosphorylation strategy, peptide-level fractionation, phosphopeptide enrichment, and data-independent acquisition mass spectrometry (DIA-MS).

Freshness

Last updated 2026-04-21 07:43:47; freshness should be verified.