N-Phosphoproteome of Nasopharyngeal Carcinoma with 1,764 Novel Sites

1,764 N-phosphorylation sites (493 pHis, 714 pLys, 557 pArg) were identified in human nasopharyngeal carcinoma cells using a potassium phosphoramidate-based mass spectrometry strategy. The dataset was created by Yuqiu Wang and published on figshare in April 2026. 85% of the identified sites are novel and not previously reported.

Use Cases

• Identify novel therapeutic targets based on differentially regulated N-phosphosites associated with signaling pathways.
• Study the role of N-phosphorylation in RNA splicing and chromatin remodeling based on pathway associations.
• Benchmark new phosphoproteomics analysis methods against the PAlibDIA strategy described.
• Investigate protein regulation in nasopharyngeal carcinoma based on the catalog of 1,764 specific sites.

Strengths

• Contains 1,764 specifically identified N-phosphorylation sites (493 pHis, 714 pLys, 557 pArg).
• 85% of the sites are reported as novel, indicating significant new findings.
• The PAlibDIA method described achieved superior coverage and quantification reproducibility compared to conventional methods.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect experimental bias inherent to the specific cell line and mass spectrometry protocol used.

Provenance

Source

figshare

Collection Method

Chemical phosphorylation with potassium phosphoramidate, peptide-level fractionation, phosphopeptide enrichment, and data-independent acquisition mass spectrometry (DIA-MS).

Freshness

Last updated 2026-04-21 07:43:44; freshness should be verified.