PAlibDIA: N-Phosphorylation Sites in Nasopharyngeal Carcinoma Cells

A dataset from figshare, authored by Yuqiu Wang, profiling noncanonical protein N-phosphorylation sites in human nasopharyngeal carcinoma cells. The data includes 493 pHis, 714 pLys, and 557 pArg sites identified using a potassium phosphoramidate-based strategy and data-independent acquisition mass spectrometry. The dataset was last updated on April 21, 2026.

Use Cases

• Identify novel N-phosphorylation sites in cancer cells based on the reported 493 pHis, 714 pLys, and 557 pArg sites.
• Analyze associations between N-phosphorylation and biological processes like RNA splicing and chromatin remodeling based on the description.
• Benchmark new phosphoproteomics methods against the PAlibDIA approach described for coverage and reproducibility.
• Investigate potential therapeutic targets in nasopharyngeal carcinoma based on differentially regulated N-phosphosites.

Strengths

• Contains 1,764 total N-phosphorylation sites (493 pHis, 714 pLys, 557 pArg) identified in a specific cancer type.
• 85% of the identified sites are reported as novel, indicating significant new findings.
• The method was validated for feasibility and reproducibility using N-phosphorylated bovine serum albumin.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is small at 4.8 MB, which may limit the scope of analysis.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Generated using a potassium phosphoramidate-based chemical phosphorylation strategy, peptide-level fractionation, phosphopeptide enrichment, and data-independent acquisition mass spectrometry (DIA-MS).

Freshness

Last updated 2026-04-21 07:43:43; freshness should be verified.