Rice sitl1 Mutant Data: Salinity Tolerance via Reduced Ion Uptake

A forward genetics study isolated a salt insensitive TILLING line 1 (sitl1) rice mutant from a gamma-ray irradiated population. The research, authored by Sung Don Lim of Kangwon National University, identified a mutation in the OsMGT1 gene, which reduced transport of Na+ and Mg2+ ions, conferring salinity insensitivity. Data likely includes genomic resequencing, RNA-sequencing, and ion uptake measurements from root, shoot, and xylem sap tissues.

Use Cases

• Train models to predict salinity tolerance based on ion uptake profiles mentioned in the description.
• Analyze gene expression patterns in response to salinity stress based on RNA-sequencing data.
• Study genotype-phenotype relationships for ion transport genes based on the identified OsMGT1 mutation.
• Compare ion accumulation in root and shoot tissues under stress conditions.

Strengths

• Data is derived from a controlled forward genetics approach using a gamma-ray irradiated TILLING population.
• Analysis includes multiple validation methods: whole genome resequencing, RNA-sequencing, yeast complementation, and protein localization assays.
• The study identifies a specific gene (OsMGT1) and mutation responsible for the observed phenotype.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

Kangwon National University

Collection Method

Forward genetics approach using a gamma-ray irradiated TILLING population, followed by whole genome resequencing and RNA-sequencing.