A Screening Strategy for Identifying DART Potential of 18 Botanical Case Studies

A 70.4 KB strategy document authored by Catherine Mahony and last updated on April 28, 2026. It outlines a battery of New Approach Methodologies for screening the developmental and reproductive toxicity of botanicals, using 18 selected botanicals as case studies. The work was conducted by the Botanical Safety Consortium DART Working Group.

Use Cases

• Evaluating the suitability of human induced pluripotent stem cell assays for botanical toxicity screening based on the described methodology.
• Benchmarking zebrafish embryo or C. elegans assays against known botanical DART hazards like locoweed or poison hemlock.
• Applying transcriptomics with Connectivity Map analysis to identify mechanistic pathways for botanical mixtures with uncertain effects.
• Testing in silico tools for predicting DART potential of complex botanical supplements mentioned in the review.

Strengths

• Strategy is based on a review of multiple relevant NAMs, including human iPSC, zebrafish, and C. elegans assays.
• Case studies include 18 botanicals selected to represent clear positives, suspected positives, and low-concern examples.
• Document is openly licensed under CC-BY-4.0, permitting reuse and adaptation.

Limitations

• The dataset is a 70.4 KB DOCX strategy paper, not a primary data collection, which limits direct analysis.
• Row count and column-level documentation are absent; the file contains textual review content.
• Freshness should be verified as the last update date is in the future (2026-04-28).

Provenance

Source

figshare, authored by Catherine Mahony, associated with the Botanical Safety Consortium DART Working Group.

Collection Method

A review and strategic outline of selected New Approach Methodologies and botanical case studies.

Freshness

Last updated 2026-04-28 16:20:03