Universal Buffer System for Native LC-MS Analysis of Antibody Therapeutics

Fluorinated ethylamines provide a volatile buffer system for native liquid chromatography-mass spectrometry (LC-MS) across a 4.5–9.8 pH range. The 122.6 KB dataset, authored by Kent R. Vosper and last updated in March 2026, likely contains results comparing these buffers to traditional nonvolatile ones for analyzing monoclonal antibodies and antibody-drug conjugates. This work demonstrates the system's applicability across major chromatographic modalities like ion-exchange and hydrophobic interaction chromatography.

Use Cases

• Benchmarking volatile buffer performance against traditional nonvolatile buffers based on elution profiles and peak broadening mentioned in the description.
• Resolving charge variants of monoclonal antibodies using volatile pH or ionic strength gradients described in the study.
• Analyzing drug-to-antibody ratio (DAR) species of antibody-drug conjugates via hydrophobic interaction chromatography in volatile phases.
• Comparing innovator and biosimilar antibody separations to assess method reproducibility with the proposed buffer system.

Strengths

• Dataset is focused on a specific, novel analytical method for native LC-MS using fluorinated ethylamine buffers.
• Results are demonstrated across multiple chromatography modalities (ion-exchange, HIC) and for different analytes (mAbs, BSA, ADCs).
• Data is shared under a CC-BY-NC-4.0 license, permitting non-commercial reuse with attribution.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The 122.6 KB size indicates a very small dataset, likely containing summary results rather than raw instrument data.

Provenance

Source

figshare, authored by Kent R. Vosper.

Collection Method

Likely contains experimental results from evaluating fluorinated ethylamine buffers for native LC-MS analysis.

Time Range

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Freshness

Last updated 2026-03-18 02:14:44; freshness should be verified.

Geography

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