CRISPR/Cas9 Genome Editing Results for Acremonium chrysogenum

Acremonium chrysogenum is a fungus used for industrial cephalosporin antibiotic production. Zhen Chen developed a tRNA-gRNA array-based CRISPR/Cas9 multiplex genome-editing system for this organism, enabling gene knockout, large-fragment deletion, and overexpression. The dataset, last updated in April 2026, likely contains experimental results from this system.

Use Cases

• Benchmark CRISPR/Cas9 editing efficiency based on promoter comparisons described in the study
• Analyze the impact of multiplex gene editing on cephalosporin C production titers
• Study the feasibility of large-fragment DNA deletion in industrial fungal strains
• Compare endogenous tRNA promoter performance against heterologous promoters for fungal genome editing

Strengths

• Includes specific efficiency metrics, such as a 95.5% knockout efficiency for the tRNAVal promoter
• Reports a successful 50.7-kb DNA fragment deletion with nearly 100% efficiency
• Demonstrates a concrete application outcome, increasing CPC titer by 23.8% from 5.59 g/L to 6.92 g/L

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The dataset is very small (88.1 KB), indicating limited scope

Provenance

Source

figshare

Collection Method

Likely contains experimental data from a CRISPR/Cas9 genome-editing study.

Freshness

Last updated 2026-04-29 11:20:50; freshness should be verified