Oligonucleotide Fragmentation Data from Multiple Ion Activation Experiments

Figshare hosts experimental data on oligonucleotide fragmentation using various ion activation methods on a timsOmni mass spectrometer. The dataset, authored by Frédéric Rosu and last updated in April 2026, includes results from techniques like electron detachment dissociation (EDD) and collision-induced dissociation on sequences including a therapeutic oligonucleotide and a 46-mer DNA/RNA. The file is 131.9 KB in size and is shared under a CC-BY-NC-4.0 license.

Use Cases

• Comparing fragmentation efficiency of different ion activation methods (EDD, CID, IRMPD, UVPD) based on the described experiments.
• Analyzing sequence coverage for oligonucleotide therapeutics like Fomivirsen based on the electron detachment dissociation results.
• Studying fragmentation patterns of long nucleic acid sequences (46-mer DNA/RNA) at low charge states as mentioned in the description.
• Validating the formation of specific ion fragments (d from a•, w from z•) from MS⁴ experiments described in the dataset.

Strengths

• Includes data from four distinct ion activation techniques (RCID, EDD, IRMPD, UVPD) as explicitly listed.
• Covers a range of oligonucleotide samples, including a therapeutic (Fomivirsen) and long sequences (46-mer DNA and RNA) as described.
• Demonstrates multi-stage (MS³, MS⁴) experimental combinations such as EDD-RCID.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (131.9 KB), indicating limited scope or a focused experimental subset.

Provenance

Source

Figshare, author Frédéric Rosu.

Collection Method

Experimental data from ion activation experiments on a timsOmni mass spectrometer with an Omnitrap platform.

Time Range

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Freshness

Last updated 2026-04-30 14:33:30; freshness should be verified.

Geography

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