RALP1 Study: Primer Sequences for CRISPR/Cas9 Gene Editing in Malaria Parasites

A 11.5 KB Excel file published by Jing Wu on figshare in April 2026. It contains primer sequences designed for CRISPR/Cas9-mediated genetic modification of the RALP1 gene in the malaria parasite Plasmodium falciparum. The data supports the specific study investigating the gene's role in schizont maturation and erythrocyte invasion.

Use Cases

• Designing homologous recombination experiments based on the provided primer sequences for the ralp1 gene.
• Verifying genomic integration events in transgenic parasites using the diagnostic PCR primers listed.
• Replicating or adapting the CRISPR/Cas9 gene editing protocol described in the associated research.

Strengths

• File size is 11.5 KB, indicating a concise and focused dataset.
• Published under a permissive CC-BY-4.0 license for reuse.
• Associated with a specific, peer-reviewed research study on a critical malaria gene.

Limitations

• Row count and column definitions are unknown, requiring manual inspection after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Primers were designed for a specific CRISPR/Cas9 experiment, likely synthesized in vitro.

Time Range

Associated with the research publication date (likely 2026).

Freshness

Last updated 2026-04-15 03:32:39; freshness should be verified.

Geography

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