TCOF1 Gene Expression and Proteomics Data in Renal Cancer Cell Lines

Supplementary data from a study on the TCOF1 gene's role in renal cancer angiogenesis. The dataset includes qPCR primer sequences, statistical analysis of protein expression changes across 169 normal kidney samples and 219 ccRCC tumor samples, microarray results from 3 biological experiments, and proteomics results from 4 biological experiments. Data was deposited in NCBI GEO (GSE299580) and ProteomeXchange (PXD027601) by author Małgorzata Grzanka.

Use Cases

• Analyze TCOF1 protein expression significance across different ccRCC tumor grades based on the statistical data.
• Compare gene expression profiles in silenced TCOF1 cells using the microarray analysis results.
• Identify protein-level changes resulting from TCOF1 silencing using the proteomics analysis data.
• Validate qPCR experiments using the provided primer sequences.

Strengths

• Includes protein expression data for 169 normal kidney samples and 219 ccRCC tumor samples across four grades.
• Microarray and proteomics analyses are backed by 3 and 4 independent biological experiments respectively.
• Data is publicly archived in NCBI GEO and ProteomeXchange repositories.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is specific to 786-O and Caki-1 cell lines, limiting generalizability.

Provenance

Source

Data retrieved from UALCAN/CPTAC and generated from cell culture experiments.

Collection Method

qPCR, microarray, proteomics, and statistical analysis of publicly available and experimental data.

Freshness

Last updated 2026-04-24 03:37:40; freshness should be verified.