Supplementary Microscopy Images and Plasmids for PRV-Induced Tunneling Nanotube Study

Supplementary material 1 contains microscopy images and plasmid validation data from a study on pseudorabies virus (PRV) infection in neuronal cells. The 17.4 MB ZIP file includes figures confirming plasmid constructs, Western blot results, and images of tunneling nanotubes (TNTs) induced by PRV strains. Author Yan Kuang uploaded this data to figshare in April 2026.

Use Cases

• Validate plasmid constructs for PRV-US3 and kinase-inactivated US3 genes using PCR, enzyme digestion, and sequencing data from S1 Fig.
• Analyze Western blot images from S1 Fig. and S1f Fig. to confirm US3 protein expression levels in different plasmid constructs.
• Study the morphology of tunneling nanotubes (TNTs) in differentiated SK-N-SH, SH-SY5Y, and mouse primary neurons using the microscopy images in S2 and S3 Figs.
• Compare PRV US3 amino acid sequences between Ea and hSD-1/2019 strains using the alignment data provided in S4 Fig.

Strengths

• Includes specific plasmid validation data, such as a 1164 bp target fragment and 6249 bp vector fragment confirmed by double enzyme digestion.
• Provides visual confirmation of neuronal markers TUBB3 and MAP2 expression in differentiated cells.
• Contains comparative Western blot results showing correct protein size expression for multiple US3 plasmid variants.

Limitations

• The dataset is small at 17.4 MB, consisting only of supplementary figures without the primary experimental data or raw image files.
• No quantitative data or tabular measurements are provided; analysis is limited to qualitative image interpretation.
• The sample data is unavailable, and the exact number of images or data points within the ZIP file is unknown.

Provenance

Source

figshare, uploaded by author Yan Kuang.

Collection Method

Generated as supplementary material for a virology research study, including experimental imaging and molecular biology validation techniques.

Freshness

Last updated on April 8, 2026, indicating recent availability.