GLYATL1 Knockout RNA-Seq Differential Expression in Luminal Breast Cancer Cells

Supplementary Table 3 provides differential gene expression analysis for long-term estrogen-deprived (LTED) MCF7 breast cancer cells and two GLYATL1 knockout clones. The 4.8 MB XLSX file contains log2 fold changes and p-values for all identified ENSEMBL/HGNC genes, comparing each knockout clone to the LTED parent line. Janina Müller authored this dataset, which was last updated on April 30, 2026.

Use Cases

• Identify genes dysregulated upon GLYATL1 knockout based on log2 fold change and p-value data.
• Validate potential biomarkers for endocrine resistance in breast cancer based on differential expression patterns.
• Compare transcriptional consequences between two distinct GLYATL1 knockout clones (KO1 and KO2).

Strengths

• Data is structured in a 4.8 MB XLSX file with separate sheets for each knockout clone comparison.
• Analysis includes both non-adjusted and FDR-adjusted p-values for rigorous statistical assessment.
• Raw sequencing data is traceable via the provided GHGA dataset ID (GHGAD23940263267179).

Limitations

• Row count and specific column names are unknown, limiting pre-download suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect experimental bias inherent to the specific MCF7 LTED cell line model.

Provenance

Source

figshare

Collection Method

RNA-sequencing of MCF7 LTED cells and LTED GLYATL1 knockout clones, with differential expression analysis performed using DESeq2 v1.28.1.

Freshness

Last updated 2026-04-30 04:13:32; freshness should be verified.