GLYATL1 Study: Acyl-CoA Metabolite Levels in Luminal Breast Cancer Cell Lines

Supplementary Table 9 provides steady-state levels of acyl-CoA species measured in MCF7 parental and long-term estrogen deprived (LTED) cells, including LTED GLYATL1 knockout clones. The dataset contains mass spectrometry peak intensities for various metabolites, normalized by internal standard levels, with five replicates per condition. Janina Müller authored this dataset, which was last updated on 2026-04-30.

Use Cases

• Compare acyl-CoA metabolite profiles between parental and estrogen-deprived breast cancer cells based on the described cell line conditions.
• Investigate the metabolic role of the GLYATL1 gene in endocrine resistance based on data from knockout clones.
• Validate mass spectrometry quantification methods for acyl-CoA species using the described normalization and identification protocols.

Strengths

• Data includes five biological replicates (n=5) per indicated condition, providing a basis for statistical analysis.
• Metabolite identification was supported by fragmentation patterns, as referenced in the description.
• Peak intensities are normalized by their respective internal standard levels, a standard practice for quantitative metabolomics.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 12.6 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Metabolites were quantified using mass spectrometry; peaks were integrated using El-MAVEN software and identified using an in-house library.

Freshness

Last updated 2026-04-30 04:13:34; freshness should be verified.