Additional File 2: Drosophila Ubiquitin Pathway and Chromatin Insulator Assay Results

Additional file 2 contains eight supplementary tables (S1-S8) from a study on the gypsy chromatin insulator in Drosophila. The tables include Z-scores for GFP foci, mass spectrometry results from CP190 and Su(Hw) pull-downs, luciferase assay p-values, ChIP-qPCR results, and primer lists. The dataset was authored by Shue Chen and last updated on 2026-05-28.

Use Cases

• Analyze the effect of ubiquitin pathway-related gene knockdowns based on Z-scores for GFP foci number, cell number, and intensity.
• Identify protein interactors based on mass spectrometry results from CP190 and Su(Hw) pull-down experiments.
• Validate chromatin insulator activity based on luciferase assay p-values for knockdowns and controls.
• Quantify protein-DNA binding based on the percent of input DNA precipitated by ChIP-qPCR results.

Strengths

• Dataset is 2.9 MB in size, making it a small and manageable download.
• Data is provided in an XLS (Excel) format, which is widely accessible for analysis.
• License is CC-BY-4.0, allowing for broad reuse and sharing.
• Contains results from multiple experimental techniques (mass spectrometry, luciferase assay, ChIP-qPCR).

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale analyses.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Experimental results from Drosophila embryonic nuclear extract assays, mass spectrometry, and molecular biology techniques.

Freshness

Last updated 2026-05-28 04:37:55; freshness should be verified.