Additional File 2: SDS-PAGE Analysis of Recombinant Tuberculosis Antigens

300 ng of purified protein per lane was analyzed for 11 antigen candidates expressed in both E. coli and Mycolicibacterium smegmatis. Eomseob Jang published this supplementary data on figshare in May 2026. The Excel file contains SDS-PAGE gel lane assignments for proteins like MPB63, MPB70, and LprG.

Use Cases

• Compare protein purity and yield between E. coli and M. smegmatis expression hosts based on the lane-by-lane SDS-PAGE analysis.
• Select optimal antigen candidates for antibody-based detection tests based on the expression success shown for each protein.
• Validate recombinant protein production methods for tuberculosis research based on the Coomassie-stained gel data.

Strengths

• Each lane contains a precisely controlled 300 ng protein load, enabling quantitative comparison.
• The dataset includes 11 distinct antigen candidates, each expressed in two different hosts.
• The data is published under a CC-BY-4.0 license, allowing for open reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is a single 692.1 KB supplementary file, offering a limited scope focused solely on gel analysis.

Provenance

Source

figshare

Collection Method

SDS-PAGE analysis of purified recombinant proteins.

Freshness

Last updated 2026-05-09 03:44:25; freshness should be verified.