Time-Lapse Microscopy Videos of Viral Transport via Tunneling Nanotubes

Eight supplementary microscopy videos document the formation and function of tunneling nanotubes in neuronal cells infected with pseudorabies virus. Videos contain 11 to 120 frames captured over durations from 120 to 600 minutes, showing filament formation and viral particle transport. The dataset was authored by Yan Kuang and uploaded to figshare in April 2026.

Use Cases

• Quantify TNT formation dynamics over time by analyzing frame sequences from S1, S2, and S3 Videos.
• Track the speed and direction of mCherry-labeled virion transport through tubular structures using frame data from S6 and S8 Videos.
• Analyze the movement patterns of multiple vesicular structures within TNTs across 68 frames in S7 Video.
• Compare EGFP transport efficiency mediated by different US3 plasmid constructs (hSD-US3 vs Ea-US3) using S4 and S5 Videos.
• Measure the stability and lifetime of long filaments from the 11-frame sequence captured over 150 minutes in S2 Video.

Strengths

• Eight distinct video files provide multiple experimental perspectives on TNT dynamics.
• Frame counts for each video are specified, ranging from 11 to 120 frames per sequence.
• Temporal resolution is documented, with frames captured at intervals of 5, 10, or 15 minutes.

Limitations

• Sample size is limited to SK-N-SH neuronal cell line observations.
• No quantitative measurements or annotations are provided alongside the raw video files.
• The dataset is a supplementary material file, not a primary, standalone research dataset.

Provenance

Source

Supplementary material from a research article on pseudorabies virus and tunneling nanotubes, authored by Yan Kuang.

Collection Method

Data gathered via inverted fluorescence microscopy (EVOS FL Auto) and confocal microscopy (ZEISS LSM 800) of infected or transfected SK-N-SH cells.

Time Range

Observation periods for individual videos range from 120 to 600 minutes.

Freshness

Data was last updated in April 2026.