Recombinant Protein Expression Conditions for Bovine Tuberculosis Detection

Additional file 3 summarizes expression conditions for recombinant proteins used in a study on antibody-based detection of bovine tuberculosis. The dataset, authored by Eomseob Jang and last updated on 2026-05-09, is a 13.0 KB XLSX file. It details host strains, expression vectors, culture media, incubation conditions, antibiotic selection, IPTG concentrations, and protein localization for proteins expressed in E. coli and Mycolicibacterium smegmatis.

Use Cases

• Compare protein expression efficiency based on host strains and vectors detailed in the description.
• Optimize culture media and incubation conditions for recombinant protein production based on the summarized parameters.
• Select appropriate antibiotic selection markers and IPTG concentrations for protein induction experiments.
• Analyze protein localization patterns (e.g., cytoplasmic, secreted) for different expression systems.

Strengths

• Dataset is small (13.0 KB), facilitating quick download and inspection.
• Specific experimental parameters like host strains, vectors, and IPTG concentrations are documented.
• Released under a permissive CC-BY-4.0 license, allowing for broad reuse.

Limitations

• Row count is unknown, which may limit suitability assessment for statistical analysis.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is a supplementary file with a limited scope focused on specific experimental conditions.

Provenance

Source

figshare

Collection Method

Summarized from a study evaluating recombinant proteins for antibody-based detection.

Freshness

Last updated 2026-05-09 03:44:25; freshness should be verified.