Primer Sequences for Cloning M. Bovis Genes for Recombinant Expression

Additional file 4 provides primer sequences used for cloning target genes from M. bovis BCG Tokyo 172 genomic DNA. The 13.3 KB XLSX file, authored by Eomseob Jang and last updated in May 2026, includes details on restriction enzyme sites and codon-optimized synthetic constructs. It supports research into recombinant protein expression in E. coli and Mycolicibacterium smegmatis for antibody-based detection of bovine tuberculosis.

Use Cases

• Designing cloning experiments based on provided primer sequences and restriction enzyme sites.
• Verifying or replicating the construction of recombinant expression vectors for M. bovis genes.
• Analyzing primer design strategies for introducing specific point mutations, as described for LprA and LppX.

Strengths

• File size is precisely 13.3 KB, indicating a focused and manageable dataset.
• Primer sequences include specific annotations like underlined restriction sites and enzyme names, adding clarity.
• The dataset is licensed under CC-BY-4.0, permitting open sharing and adaptation.

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale analyses.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset's scope is limited to primer sequences for a specific set of genes and cloning methods.

Provenance

Source

figshare, authored by Eomseob Jang.

Collection Method

Primer sequences were designed for cloning genes from M. bovis BCG Tokyo 172 genomic DNA, with some templates being codon-optimized synthetic constructs from GenScript.

Freshness

Last updated 2026-05-09 03:44:25; freshness should be verified.