Comparative Analysis of Original and Re-assembled Metagenome-Assembled Genomes

Microbiome Datahub provides a targeted comparison of twenty original and re-assembled Metagenome-Assembled Genomes (MAGs) from the same BioProjects and BioSamples. The dataset, authored by Hiroshi Mori and last updated in April 2026, reports metrics like average nucleotide identity, completeness, contamination, genome size, and contig number. It is a small, 14.7 KB Excel file summarizing results from three databases: Microbiome Datahub, MGnify, and SPIRE.

Use Cases

• Benchmarking genome assembly quality based on reported completeness and contamination metrics.
• Comparing database-specific MAG reconstructions based on average nucleotide identity (ANI) values.
• Identifying cases where corresponding MAGs are absent in specific databases, as indicated by N.D. entries.

Strengths

• Provides a direct, targeted comparison of 20 specific MAGs across three databases.
• Includes five specific quality and similarity metrics per MAG: ANI, completeness, contamination, genome size, and contig number.
• Explicitly notes cases where corresponding data is not found (N.D.), clarifying comparison scope.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset's scale is limited to 14.7 KB, representing a focused comparison rather than a broad survey.

Provenance

Source

figshare, associated with the Microbiome Datahub platform.

Collection Method

Comparative analysis of MAGs deposited in INSD and curated/reconstructed by Microbiome Datahub, MGnify, and SPIRE.

Freshness

Last updated 2026-04-23 04:42:09; freshness should be verified.