Digitalis Purpurea Genotyping PCR Designs for ANS and TFL1/CEN Loci

Additional file 6 from a genome sequencing study details the design of genotyping PCRs for two specific genetic loci. The 1.2 KB text file, authored by Jakob Maximilian Horz and last updated in May 2026, provides protocols likely used to investigate flower color and morphology variation in the ornamental plant Digitalis purpurea. Its small size suggests it contains specific primer sequences and experimental parameters rather than raw genetic data.

Use Cases

• Replicate genotyping assays for the ANS locus based on the described PCR design.
• Validate genetic markers for flower color variation in Digitalis purpurea based on the TFL1/CEN locus protocols.
• Design comparative genotyping studies for other plant species using the provided PCR methodology.

Strengths

• File is small (1.2 KB), ensuring quick download and easy inspection.
• Released under a permissive CC-BY-4.0 license, allowing for broad reuse and modification.
• Directly associated with a published genome sequence study, providing academic context.

Limitations

• The dataset is extremely small (1.2 KB), indicating very limited scope and content.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for computational analysis.

Provenance

Source

figshare, authored by Jakob Maximilian Horz.

Collection Method

Likely generated as supplementary material for a genome sequencing and analysis study.

Freshness

Last updated 2026-05-02 03:16:35; freshness should be verified.