ESAT-6 and CFP-10 Fusion Protein Characterization for Bovine Tuberculosis Detection

677.6 KB of data characterizing ESAT-6 and CFP-10 fusion proteins under native and denaturing conditions. The dataset, authored by Eomseob Jang and last updated in May 2026, includes results from Native-PAGE and Western blot analyses using anti-6X His-tag antibodies. It compares antigens expressed in E. coli and M. smegmatis.

Use Cases

• Compare protein expression and purification yields based on the host organism (E. coli vs. M. smegmatis) mentioned in the description
• Analyze protein migration patterns under native and denaturing conditions based on Native-PAGE and Western blot data
• Validate antibody detection sensitivity based on the described Western blot results using different protein loadings

Strengths

• Data is licensed under CC-BY-4.0, allowing for open sharing and reuse.
• Specific experimental details are provided, such as protein loadings (e.g., 300 ng, 500 ng, 1 µg) and staining methods (Coomassie Brilliant Blue).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for statistical analysis.
• The dataset is small (677.6 KB), indicating limited scope.

Provenance

Source

figshare

Collection Method

Experimental data from Native-PAGE and Western blot analyses.

Freshness

Last updated 2026-05-09 03:44:26; freshness should be verified.