Amnis Memory Phenotyping Expt: T Cell and Tumor Cell Imaging with Blinatumomab

1630784279 bytes of imaging flow cytometry data capture T cell and target cell interactions with and without the bispecific T cell engager blinatumomab. The dataset contains images and derived measurements from an Amnis ImageStreamX Mk II instrument, analyzing co-expression of markers like CD4, CD8, CCR7, and CD45RA on T cells and CD20 on target cells. Image analysis was performed using IDEAS software to identify in-focus cellular events and cell complexes.

Use Cases

• Quantifying T cell and tumor cell conjugate formation based on co-expression of fluorescent markers.
• Analyzing memory T cell subset phenotypes (e.g., naive, central memory) using surface markers CD45RA and CCR7.
• Evaluating the effect of blinatumomab treatment on cell-cell interaction dynamics.
• Developing image analysis pipelines for identifying cell complexes from high-content imaging flow cytometry data.

Strengths

• Data is openly licensed under CC-BY-4.0, facilitating reuse and sharing.
• Detailed, reproducible experimental protocol is provided in the description.
• Data was collected on a calibrated Amnis ImageStreamX Mk II instrument using multiple laser lines, suggesting high-quality, multi-parameter imaging.

Limitations

• Column names and the exact number of data rows are not specified, limiting understanding of the data structure.
• The dataset appears to be a single experimental snapshot with no explicit time range for data collection.
• File formats (RIF, CTM, AST) are listed but their specific contents and relationships are not described.

Provenance

Source

The University of Melbourne, author Joanne Davis.

Collection Method

Experimental data from imaging flow cytometry of co-incubated T cells and target cells, with image analysis via IDEAS software.

Freshness

Last updated 2026-03-26.