Amnis Phalloidin Immune Synapse Experiment: Imaging Flow Cytometry Data

This dataset contains imaging flow cytometry data from a cell conjugation assay investigating immune synapse formation. The data was generated using an Amnis ImageStreamX Mk II cytometer, with samples stained for F-actin using Phalloidin Alexa Fluor 488 to detect synapses between T cells and tumor cells. Image analysis was performed using IDEAS software, and the dataset includes files in CTM, RIF, DAF, and AST formats.

Use Cases

• Quantifying immune synapse formation based on phalloidin staining intensity and localization.
• Analyzing cell conjugation complexes by co-expression of T cell and tumor cell line markers.
• Validating bispecific T cell engager (BiTE) mechanisms of action through imaging cytometry.
• Developing image analysis pipelines for IDEAS software using raw imaging flow cytometry files.

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting broad reuse.
• Data was acquired on a high-content Amnis ImageStreamX Mk II imaging flow cytometer.
• Detailed, reproducible experimental protocol is provided in the description.

Limitations

• Column names and row counts are not provided, limiting understanding of data structure.
• The dataset consists of a single 280 MB file bundle; the internal file count and organization are unknown.

Provenance

Source

The University of Melbourne, author Joanne Davis.

Collection Method

Generated from a laboratory conjugation assay, with samples fixed, stained for F-actin, and analyzed via imaging flow cytometry.

Freshness

Last updated on 2026-03-26.