Anaerobic Fluorescent Reporter and CRISPR-Cas12a Data for Clostridium butyricum

A 79.29% increase in aldehyde dehydrogenase enzyme activity demonstrates the enhanced catalytic capacity for acetaldehyde oxidation achieved through metabolic engineering. The dataset contains results from establishing an anaerobic fluorescent reporter system and CRISPR-Cas12a editing platform for Clostridium butyricum. It was authored by Diying Li and last updated in April 2026.

Use Cases

• Analyze promoter strength dynamics using time-resolved transcriptomic data from native promoters.
• Compare enzyme activity levels, such as the 79.29% increase in aldehyde dehydrogenase (ALDH) activity, across engineered strains.
• Evaluate the efficiency of the conjugation method and CRISPR-Cas12a platform for scarless gene manipulation metrics.
• Screen genetic part performance using quantitative data from the anaerobic fluorescent reporter system.

Strengths

• Dataset includes a demonstrated 79.29% increase in a key enzyme activity (ALDH).
• Provides data for a complete genetic toolkit including conjugation, reporter, and CRISPR systems.
• Results are generated under strict anaerobic conditions, a key technical hurdle.

Limitations

• Dataset size is small at 1.9 MB, suggesting limited raw data volume.
• Unknown row count and specific column structure limits immediate analysis scope.
• Data is specific to Clostridium butyricum, limiting generalizability to other organisms.

Provenance

Source

Diying Li via figshare.

Collection Method

Experimental data from establishing genetic tools and metabolic engineering in Clostridium butyricum.

Freshness

Last updated April 2026.