Sorghum and Foxtail Millet RNA-seq Data for Time-of-Day Cold Responses

Illumina NovaSeq X Plus sequencing data from RNA samples extracted from sorghum and foxtail millet leaf tissue collected after cold exposure at dawn, dusk, or under control conditions. Three replicates were collected, each representing an independent growth trial under a 14-hour light/10-hour dark photoperiod. FASTQ files are available through the European Nucleotide Archive, with two foxtail millet samples and one sorghum sample excluded due to poor quality control.

Use Cases

• Identify cold-responsive genes based on RNA expression changes after dawn or dusk exposure.
• Compare circadian regulation of cold tolerance between sorghum and foxtail millet.
• Analyze the impact of photoperiod on plant stress physiology using the 14-hour light/10-hour dark growth condition.
• Validate RNA-seq quality control metrics using the excluded samples as a reference for failure thresholds.

Strengths

• Sequencing performed on the Illumina NovaSeq X Plus platform with a target of approximately 20 million read pairs per sample.
• Three biological replicates collected, each representing an independent growth trial.
• Data is structured around a controlled experimental design with specific time points (dawn, dusk, control) and a defined photoperiod.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Two foxtail millet samples and one sorghum sample were excluded, reducing the complete sample set.

Provenance

Source

figshare

Collection Method

poly(A)-enriched RNA sequencing of leaf tissue from plants grown under controlled photoperiod and cold exposure.

Freshness

Last updated 2026-05-21 19:45:56; freshness should be verified.