Cysteine Ligandability Data Comparing Live-Cell and Lysate-Based ABPP Platforms

Ka Yang's dataset compares cysteine ligandability measurements between live-cell and native-lysate activity-based proteome profiling (ABPP) platforms. The 45.8 MB XLSX file, last updated in May 2026, provides a framework for ABPP experimental design and data interpretation. It highlights factors like protein relocalization and the effects of the CDK7 inhibitor THZ1.

Use Cases

• Comparing cysteine ligandability measurements based on live-cell versus lysate-based ABPP platforms.
• Analyzing protein relocalization effects based on data from CDK7 inhibitor THZ1 treatment.
• Identifying confounding factors in proteomics data based on discrepancies from cysteine accessibility, protein abundance, and relocalization.

Strengths

• Dataset size is 45.8 MB, indicating a substantial amount of data.
• The description provides a clear, specific research context comparing two ABPP platforms and identifying three major confounding factors.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The description is specific to a biochemical study, which may limit generalizability to other domains.

Provenance

Source

figshare

Collection Method

Generated via a high-throughput TMT-ABPP workflow adapted for live cells and compared to native lysates.

Freshness

Last updated 2026-05-29 10:29:37; freshness should be verified.