Anisodine-Protein Interactions from HuProt Human Proteome Microarray

2,877 positive proteins and 729 specific positive proteins were identified from a HuProt human proteome microarray chip experiment comparing biotin-labeled anisodine hydrobromide to a D-biotin control. The dataset includes raw chip data, analysis results, scanned images, and project documentation. Anqing Li published the dataset on figshare under a CC-BY-4.0 license, with a last update in 2026.

Use Cases

• Identify potential protein targets for the small molecule anisodine based on the reported 729 specific positive proteins.
• Perform comparative statistical analysis of protein binding using the provided control sample (D-biotin) data.
• Validate microarray chip image analysis techniques using the provided raw chip images and extracted data.
• Cross-reference identified protein interactions with external databases using the provided HuProt V4.0 protein information file.

Strengths

• Includes raw data files (Excel), processed analysis results, and scanned chip images (JPG), providing a multi-format view of the experiment.
• Reports specific quantitative results: 2,877 total positive proteins and 729 specific positive proteins for the Ani-biotin sample.
• Provides a control sample dataset (D-biotin) for comparative statistical analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count for the raw and processed data files is unknown, which may limit suitability assessment.
• The description metadata is limited; actual data quality and experimental protocol details require manual inspection of the provided Word documents.

Provenance

Source

figshare

Collection Method

Data generated from a HuProt human proteome microarray chip experiment comparing anisodine-biotin and D-biotin samples.

Freshness

Last updated 2026-04-23 22:32:46; freshness should be verified.