RNA extraction data from various tissues of the marine mussel Mytilus galloprovincialis, including gonads and somatic tissues like gills and muscle. Adult mussels were commercially obtained from fishers along the Algarve coast, dissected, and sexed based on gonad appearance. Total RNA was extracted, quality-assessed with a Nanodrop, and treated with DNase for cDNA synthesis for PCR and downstream experiments by author Heather Marco.
Use Cases
- Differentiating between male and female mussels based on RNA extracted from various tissues.
- Studying gene expression changes across different times of the reproductive cycle using RNA data.
- Conducting PCR and quantitative PCR analyses on cDNA synthesized from mussel tissue RNA.
- Comparing RNA profiles between gonadal and somatic tissues such as gills, foot muscle, neural tissues, and digestive tissues.
Strengths
- Includes RNA from multiple tissue types (gonads, gills, foot muscle, neural tissues, digestive) per animal.
- Sex of specimens was confirmed through histological examination of gonads.
- RNA quality was assessed with a Nanodrop and treated with DNase to remove DNA contamination.
Limitations
- Row count is unknown, which may limit suitability assessment.
- Column-level documentation is absent; field semantics must be inferred after download.
- Data may reflect geographic bias inherent to specimens sourced from the Algarve coast.
Provenance
- Source
- Heather Marco via figshare
- Collection Method
- RNA extraction from dissected mussel tissues using a kit, followed by quality assessment and DNase treatment.
- Freshness
- Last updated 2026-04-28 09:53:29; freshness should be verified.
- Geography
- Algarve coast