African Turquoise Killifish Brain Atlas Confocal Validation Images

Maximum z-projected confocal microscopy images for spatial validation of a brain atlas in the African turquoise killifish. The dataset includes images from 4-plex cell-type labeling and differentially expressed gene sets, captured using a Leica Stellaris 5 confocal microscope. It was authored by Rapheal Williams and last updated in April 2026.

Use Cases

• Validate spatial co-localization of cell-type markers like apoeb, mpz, s100b, and map2 within the optic tectum brain region.
• Analyze expression patterns of differentially expressed genes such as znfx1, helz2, ifih1, optn, irf3, and stat1a from multi-channel fluorescent images.
• Develop image segmentation models using DAPI (Channel 1) for nucleus detection across 1024x1024 and 512x512 resolution images.
• Correlate anatomical structures guided by a coronal brain atlas with fluorescence intensity from specific fluorophores like TSA Vivid Fluorophore 520, 570, Opal 620, and Opal 690.

Strengths

• High-resolution imaging with 1024x1024 and 512x512 pixel resolutions.
• Multi-channel data capturing up to 5 distinct fluorescent markers per image.
• Detailed acquisition metadata including laser intensities (10), digital gain (2.5%), and microscope objectives (10x/0.4, 63x/1.4).

Limitations

• Raw volumetric data (.ims/.lif files) are not included, only compressed, merged-channel maximum z-projections.
• Sample size and number of brain slices are not specified in the provided description.
• Geographic or strain-specific origin of the African turquoise killifish samples is not detailed.

Provenance

Source

Figshare, authored by Rapheal Williams.

Collection Method

Imaging via Leica Stellaris 5 confocal microscope with 10X dry and 63x oil-immersion objectives; samples prepared with in situ hybridization and fluorescent probes.

Freshness

Last updated in April 2026.