Audet et al. 2026: Drosophila Foreleg Development RNA-Seq Data and Analysis Scripts

A 64.0 MB repository of intermediate data and analysis scripts supporting a 2026 study on the genomic architecture of foreleg development in Drosophila prolongata. The dataset includes gene read counts for three Drosophila species (prolongata, carrolli, melanogaster) and R scripts for differential expression and PCA analysis. It was authored by Tyler Audet and colleagues and published on figshare under a CC-BY-4.0 license.

Use Cases

• Perform differential gene expression analysis based on the provided R scripts and gene read count data.
• Conduct PCA analysis on RNA-seq data from three Drosophila species.
• Validate differential expression methods against standard DESeq2 workflows using the included scripts.
• Analyze phenotypic measurements from a genetic cross experiment using the provided pengal4_data.csv file.

Strengths

• Includes processed gene read count data for three Drosophila species (prolongata, carrolli, melanogaster).
• Provides complete R scripts for reproducing the paper's figures and analyses.
• Links to raw sequence data via a public NCBI GEO accession (GSE330706).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

RNA sequencing (RNA-seq) data analysis outputs from a scientific study.

Freshness

Last updated 2026-05-17 22:53:01; freshness should be verified.