Automated Middle-Down Mass Spectrometry Antibody Subunit Analysis

Proteoform Studio software workflows for automating antibody subunit analysis via middle-down mass spectrometry. The dataset, created by Amy K. Carfagno and last updated in April 2026, includes results demonstrating over 80% sequence coverage of antibody subunits. It is a small dataset of 97.6 KB stored in an XLSX file.

Use Cases

• Automating intact protein characterization workflows based on the described complete analysis pipeline.
• Training models for automated fragment ion matching based on signal-to-noise ratio and isotope fit score settings.
• Benchmarking sequence coverage results for antibody light and heavy chains based on the reported >80% coverage.
• Aggregating spectral results from complementary fragmentation methods for improved proteoform identification.

Strengths

• Demonstrates >80% sequence coverage of antibody subunits.
• Includes automated settings for fragment ion matching (signal-to-noise ratio, isotope fit score) validated against manual results.
• File format is XLSX, a widely accessible tabular format.

Limitations

• Row count and column definitions are unknown, limiting suitability assessment.
• Dataset is very small (97.6 KB), indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.

Provenance

Source

figshare

Collection Method

Likely contains results from automated mass spectrometry analysis workflows.

Time Range

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Freshness

Last updated 2026-04-13 13:36:29.

Geography

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