HPLC-UV Method for Phenylboronic Acid Analysis Using a Bromo-Analog Surrogate

A dual-standard reverse-phase HPLC-UV method was developed for the simultaneous identification, assay, and impurities analysis of phenylboronic acid. The method uses 4-methoxyphenyl boronic acid as a model compound and a bromo-analog surrogate for quantitation, bypassing the need for a single-component primary reference standard. This 1.7 MB dataset, authored by Weijiang Ying and last updated in May 2026, is available under a CC-BY-4.0 license.

Use Cases

• Validate a QC-friendly HPLC method for boronic acid analysis based on the described gradient and column parameters.
• Quantify impurities in phenylboronic acid samples using the described bromo-analog surrogate standard approach.
• Benchmark separation performance for related compounds using the specified Ascentis Express ES-Cyano column and mobile phase conditions.

Strengths

• Method details a specific injection volume (10 μL) and flow rate (0.8 mL/min).
• Employs a stable surrogate standard, which the description suggests improves practicality.
• Claims baseline separation of the analyte and its impurities, indicating a resolved chromatographic method.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (1.7 MB), suggesting it contains methodological documentation rather than large experimental data tables.

Provenance

Source

figshare

Collection Method

Likely contains the documented experimental method and validation data for the described HPLC-UV procedure.

Freshness

Last updated 2026-05-28 23:27:04; freshness should be verified.