Fasting Mouse Liver Transcriptome and Splicing Dynamics Across 36 Hours

Supplemental data files for a 2026 bioRxiv manuscript by Jessica Treeby. The 1.5 GB repository contains results from RNA-seq analyses of male and female C57BL/6 mice, sampled every 4 hours during a 36-hour fast. Raw sequencing data is available separately under NCBI GEO accession GSE328655.

Use Cases

• Identify differentially expressed genes during fasting based on DESeq2 results.
• Analyze time-course trends in gene expression based on maSigPro analysis.
• Model circadian rhythmicity in liver transcriptomes based on dryR analysis.
• Investigate alternative splicing events based on differential exon usage (DEXSeq) results.

Strengths

• Data is sex-stratified, enabling analysis of biological differences.
• High-resolution time-series sampling every 4 hours across a 36-hour fast.
• Results from multiple complementary analyses (DESeq2, maSigPro, dryR, DEXSeq) are included.
• Raw data is publicly archived under a known accession number (GSE328655).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, associated with a bioRxiv manuscript by Treeby et al. (2026).

Collection Method

RNA-seq analysis of liver tissue from C57BL/6 mice.

Time Range

Experimental time course of 36 hours.

Freshness

Last updated 2026-04-29 09:14:40; freshness should be verified.