Bovine Placenta Single-Nucleus Transcriptome Atlas Across 13 Gestational Timepoints

311,299 placental cells or nuclei were profiled across 13 developmental stages from E12 to E280 in cows. Guanghui Tan created this longitudinal atlas to identify 13 major cell types and 14 trophoblast subtypes. The dataset was published on figshare in April 2026.

Use Cases

• Model trophoblast subtype differentiation trajectories using gene expression profiles from specific uninucleate cell populations.
• Identify stage-specific expression patterns and key regulatory genes like HAND1 and DLX5 for maternal pregnancy recognition.
• Perform integrated GWAS analysis to link early trophoblast subtypes and genes like CYCS or HMGA1 with gestation length traits.
• Analyze the origin and association of placental macrophage cell types with pregnancy loss risk pathways.
• Study the co-regulation of binucleate cell differentiation by imprinted genes and metabolic reprogramming markers.

Strengths

• Profiles 311,299 single cells or nuclei, providing high-resolution cellular data.
• Covers 13 distinct developmental timepoints (E12 to E280), enabling longitudinal analysis.
• Identifies and characterizes 13 major cell types and 14 trophoblast subtypes with associated regulatory networks.

Limitations

• Sample data and specific column names are unavailable, complicating initial data exploration.
• The dataset is specific to bovine (cow) placentas, limiting direct applicability to human or other model organisms without careful translation.
• The 3.8 GB size and inclusion of multiple file formats (ZIP, PNG, PDF, TIF, CSV) may require significant effort to integrate and parse.

Provenance

Source

figshare, authored by Guanghui Tan.

Collection Method

Single-nucleus transcriptomic sequencing and bioinformatic analysis of bovine placental tissue.

Time Range

Developmental stages from embryonic day 12 (E12) to embryonic day 280 (E280).

Freshness

Last updated April 2026.

Geography

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