Arctic Tundra Soil Metagenome-Assembled Genomes from PacBio HiFi Sequencing

A genome-resolved metagenomic dataset from Arctic tundra permafrost soils at Cambridge Bay, Canada. It contains a non-redundant catalog of approximately 500 metagenome-assembled genomes (MAGs), including 14 complete, 41 near-complete, and 99 high-quality genomes. The dataset was generated from about 26 million PacBio HiFi long reads and spans diverse bacterial and archaeal lineages.

Use Cases

• Studying microbial diversity and taxonomy in Arctic permafrost based on the catalog of ~500 MAGs.
• Comparative genomic analysis of novel bacterial and archaeal lineages based on the described taxonomic novelty.
• Benchmarking metagenomic assembly and binning methods using long-read sequencing data from a complex soil environment.
• Investigating microbial adaptations to cold environments based on genomes reconstructed from active-layer soils.

Strengths

• Includes ~500 metagenome-assembled genomes (MAGs) with quality tiers (14 complete, 41 near-complete, 99 high-quality).
• Generated from ~26 million PacBio HiFi long reads (~133 Gb) with a mean read length of 5.1 kb.
• Dataset size of 17.4 GB indicates substantial genomic content.
• Spans diverse bacterial and archaeal lineages with substantial taxonomic novelty.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count for genomic sequences is unknown, which may limit suitability assessment.
• Data may reflect geographic bias inherent to the single sampling location at Cambridge Bay.

Provenance

Source

figshare

Collection Method

PacBio HiFi long-read sequencing of dry surface soils from the active layer of Arctic tundra permafrost.

Time Range

null

Freshness

Last updated 2026-05-04 04:15:38; freshness should be verified.

Geography

Cambridge Bay, Canada (Arctic tundra permafrost)