Drosophila UBE3A Substrate Data with LC-MS/MS and Western Blot Files

Supporting materials for a study on Drosophila UBE3A regulation include supplemental tables of tested fly lines and complete LC-MS/MS datasets from UBAIT experiments. The repository contains raw and processed Western blot image files and an inventory of generated plasmids and vectors. Author Benjamin Geier uploaded the 2.3 MB collection to figshare in April 2026 under a CC-BY-4.0 license.

Use Cases

• Identify potential UBE3A substrates based on the LC-MS/MS datasets from GAL4-specific UBAIT experiments.
• Validate protein interactions or expression levels using the provided raw and processed Western blot data.
• Replicate genetic constructs using the comprehensive inventory of all plasmids and vectors generated in the study.
• Screen Drosophila lines for genetic studies based on the supplemental tables listing all tested lines.

Strengths

• Includes raw LC-MS/MS data files, allowing for independent re-analysis.
• Provides original Western blot image files labeled for clarity.
• Contains a complete inventory of all plasmids and vectors generated, facilitating reproducibility.
• Released under a permissive CC-BY-4.0 license.

Limitations

• Row count and specific column definitions are unknown, requiring manual inspection after download.
• The dataset is small at 2.3 MB, which may limit the scope of analysis.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare repository associated with a research manuscript.

Collection Method

Data generated from laboratory experiments including LC-MS/MS and Western blotting.

Freshness

Last updated 2026-04-26 20:18:14; freshness should be verified.