Cold-Active Pullulanase Mutant Performance Data

A dataset from figshare contains experimental data on engineered cold-active pullulanase enzymes. The data likely includes enzyme activity measurements, kinetic parameters, and stability profiles for wild-type and truncated variants. Author 璨 汤 published the dataset under a CC-BY-4.0 license in May 2026.

Use Cases

• Analyzing enzyme thermostability improvements based on half-life extension data
• Comparing catalytic efficiency based on kcat/Km kinetic parameters
• Evaluating secretion efficiency based on extracellular enzyme activity measurements
• Benchmarking mutant performance under acidic pH conditions

Strengths

• Includes specific performance metrics, such as a 14.7-fold increase in extracellular enzyme activity
• Contains kinetic analysis data, including a reported 6.7-fold higher kcat/Km value
• Provides stability data, such as a 7-fold extension of half-life at 45 °C

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment

Provenance

Source

figshare

Collection Method

Likely contains experimental results from heterologous expression and enzyme characterization.

Freshness

Last updated 2026-05-06 14:41:43; freshness should be verified