Saccharomycotina tRNA Gene Sequences and Modification Enzyme Repertoires

Lauren Dineen's dataset provides tRNA gene sequences for 1154 Saccharomycotina yeast species, integrated with annotations for tRNA modification enzymes. It includes comparative genomic analysis and Nano-tRNAseq modification profiles for three focal species: Saccharomyces cerevisiae, Hanseniaspora uvarum, and Yarrowia lipolytica. This work offers a multi-species view of tRNA sequence conservation and enzyme repertoire variation across a eukaryotic subphylum.

Use Cases

• Comparative analysis of tRNA gene sequence conservation across 1154 yeast species.
• Annotation and investigation of tRNA modification enzyme repertoires, including lineage-specific losses.
• Linking enzyme presence/absence to tRNA gene sequence features using General Linearized modelling.
• Profiling tRNA modification signatures via Nano-tRNAseq data for three focal species.

Strengths

• Covers tRNA gene sequences for 1154 Saccharomycotina species, providing broad taxonomic scope.
• Integrates multiple data types: genomic sequences, enzyme annotations, and experimental modification profiles.
• Employs machine learning (Random Forest) and statistical modelling (General Linearized) for analysis.

Limitations

• Column names and exact row counts are not specified in the provided metadata.
• Experimental modification profiling is limited to three species (S. cerevisiae, H. uvarum, Y. lipolytica).

Provenance

Source

Lauren Dineen

Collection Method

Comparative genomic analysis, machine learning annotation, and Nano-tRNAseq experimental profiling.

Time Range

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Freshness

Last updated on 2026-04-03.

Geography

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